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For those with budget constraints preventing them from purchasing a new instrument, Fast reagents allow to reduce the bottleneck on instrument and increase its daily throughput capabilities. Thermofisher developed and validated Fast master mixes which enables you to convert from standard to Fast qPCR without reoptimizing the assays.įurthermore, if you are working with TaqMan chemistry or SYBR chemistry, TaqMan® Fast Advanced Master Mix and PowerUp™ SYBR™ Green Master Mix have been engineered to retain high level of performance in preassembled reactions for up to 72 hours which enables you to convert from standard to Fast qPCR without having to worry about the stability of master mix affecting the results when processing plates all day.įast reagents can also be a more cost-effective solution than standard cycling reagents for your real-time PCR experiments. This enables getting answers faster and process more samples during allotted time, and if anything should go wrong, using fast mode enables repeating a run during scheduled time. Running in Fast mode will triple the number of researchers or runs on that same instrument by reducing times from 120 minutes to as little as 40 minutes or even 30 minutes depending on the instrument. Under current standard cycling conditions, a typical run takes approximately 2 hours, limiting the number of researchers or runs on that instrument to only four per 8-hour day. 1 It can be used in either standard or fast cycling mode and is compatible with all qPCR instruments.ġ Dynamic range is a property of both the assay and template concentration in the sample, as well as in the master mix, thus individual results may vary.Recently, fast PCR protocols have been developed to increase assay throughput. PowerTrack™ SYBR® Green Master Mix provides robust results ranging from 100 ng to 0.1 pg of complementary DNA (cDNA) per reaction. The inclusion of UNG and dUTP enzymes also prevents previously amplified PCR products from contaminating subsequent qPCR reactions. For researchers using qPCR for gene expression analysis, specific amplification of target genes is important. PowerTrack SYBR Green Master Mix uses an antibody-mediated hot-start mechanism to provide tight control over Taq enzyme activation and help prevent early activity of the polymerase at low temperatures that can lead to nonspecific amplification.

The yellow sample buffer is added separately to indicate that sample has been added to the reaction, based on a visual color change of the reaction mix from blue to green.

The master mix contains an inert blue dye and a separate, optional yellow sample buffer. Green dye immediately binds to all double-stranded DNA. The master mix contains a two-color tracking dye system designed to help researchers keep track of where they have pipetted. When PowerUp SYBR Green Master Mix is added to a sample, SYBR. PowerUp SYBR Green Master Mix (Recommended). Coupled with user-supplied primer sets and template, PowerTrack SYBR Green Master Mix is designed to amplify targets for accurate gene expression analysis. PowerUp SYBR Green Master Mix USER GUIDE Universal 2X master mix for real-time PCR workflows Catalog Numbers A25741, A25742, A25743, A25776, A25777. Wide instrument compatibility, and the best choice for Applied Biosystems instruments. The PowerTrack SYBR Green Master Mix is a pre-formulated, universal, 2X concentration Master Mix for real-time PCR workflows with built-in visual dye–based indicators for accurate reaction set up. Applied Biosystems SYBR Green mastermixes are designed to be used in real time PCR quantitation reactions where the target is amplified using a flanking. Thermo Fisher Scientific continues to innovate its SYBR Green real-time PCR (qPCR) master mix portfolio with the launch of the Applied Biosystems™ PowerTrack™ SYBR™ Green PCR Master Mix.